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M94A1882.TXT
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1994-10-24
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Document 1882
DOCN M94A1882
TI Quantitation of plasma HIV-1 RNA by branched DNA (bDNA) signal
amplification.
DT 9412
AU Wilber J; Urdea M; Detmer J; Kolberg J; Zanki S; Todd J; Chiron
Corporation, Emeryville, CA.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):43 (abstract no. 145B). Unique
Identifier : AIDSLINE ICA10/94370693
AB OBJECTIVE: To measure HIV-1 RNA in a large number of plasma specimens
precisely, accurately, and rapidly in order to monitor HIV therapy and
to determine whether quantitation is affected by differences in HIV-1
subtype. METHODS: HIV-1 RNA levels in plasma were measured using a solid
phase nucleic acid hybridization assay (Quantiplex HIV-RNA) based on
bDNA signal amplification technology. In vitro RNA transcripts of the
pol region of HIV-1 subtypes A-E, purified and quantified by OD 260,
phosphate analysis, and hyperchromicity, 148 specimens from patients who
had > 400 CD4/microliters, and longitudinal samples from patients on
therapy were quantified using the bDNA assay. A lower clinical cutoff
was evaluated. RESULTS: All 5 distinct genetic subtypes quantified
within a factor of 1.5. One operator could complete testing of 42
specimens in duplicate in less than 24 hr. The new clinical cutoff
increased the number of specimens detectable by this assay in patients >
400 CD4 by > 20% (levels shown in Fig). DISCUSSION AND CONCLUSIONS: The
bDNA assay is a simple, reproducible, and accurate method for measuring
HIV-1 RNA regardless of subtype that may be useful in monitoring changes
in RNA level during the course of disease and during antiviral therapy.
HIV-1 RNA levels vary widely in patients with high CD4 levels,
suggesting that stratification of treatment could be based on viral load
rather than CD4 level. TABULAR DATA, SEE ABSTRACT VOLUME.
DE Antiviral Agents/THERAPEUTIC USE DNA, Viral/GENETICS Gene
Amplification Genes, pol Human HIV Infections/BLOOD/DRUG
THERAPY/MICROBIOLOGY HIV-1/CLASSIFICATION/GENETICS/*ISOLATION & PURIF
Leukocyte Count Nucleic Acid Hybridization Reproducibility of Results
RNA, Viral/*BLOOD/GENETICS T4 Lymphocytes Viremia/BLOOD/DRUG
THERAPY/MICROBIOLOGY Virology/*METHODS/STATISTICS & NUMER DATA MEETING
ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).